Dr. Carl Gordon Johnston
Molecular Biology and Microbiology
cgjohnston AT ysu DOT edu
B.S. University of Manitoba, Winnipeg, Canada, Honors Microbiology, 1981
M.S. University of Alaska, Fairbanks, Alaska, Chemical Oceanography, 1986
Ph.D. University of Cincinnati, Cincinnati, Ohio, Biology, 1992
Carl is active in the Mahoning River Consortium as an advocate for river restoration and is active in remediation and restoration research. He collaborates with faculty and students across STEM at YSU and with industry partners conducting research on bioremediation of 1) contaminated river sediment and 2) water impacted by mine waste. Bioremediation is being considered by the United States Army Corps of Engineers for restoration of the Mahoning River. His collaboration with BioRemedial Technologies Inc. (BRT), and Winner Global LLC is focused on optimizing activity of indigenous sulfate reducing bacteria to treat acid mine drainage. Carl is working towards technology scale up and full-scale bioremediation.
D'Elia, TV, CR Cooper Jr., and CG Johnston (2007). Source tracking of Escherichia coli by Denaturing Gradient Gel Electrophoresis (DGGE) of the rrnB Ribosomal Operon in the 16S-23S Intergenic Spacer Region. Can J Microbiol 53:1174-1184.
Mosher, JJ, RH Findlay, and CG Johnston (2006) Physical and chemical factors affecting microbial biomass and activity in contaminated subsurface sediment. Can J Microbiol 52:397-403
Basto Salgado, GP, TP Diggins, CG Johnston, and W Fairchild (2005) Water quality and benthic community structure in Cattaraugus Creek, Zoar Valley, NY. Hydrolog Sci Technol J
Humphries, JA, AMH Ashe, and CG Johnston (2005) Effect of Phenol on Microbial Community Structure and Trichloroethylene Degradation in Groundwater. Can J Microbiol 51:433-439
Mosher, JJ, BS Levison, and CG Johnston 2003 A simplified dehydrogenase enzyme assay in contaminated sediment using 2-(p-iodophyenyl)-3(p-nitrophenyl) tetrazolium chloride (INT). J Micro Meth. 53:411-415.
Other Publications Relevant to Bioremediation
Beltz, LA, DR Neira, CA Axtell, S Iverson, W Deaton, JA Bumpus, and CG Johnston (2001) Immuno-toxicity of explosives-contaminated soil before and after bioremediation. Arch Env Contam Tox 40:311-317.
Axtell, C, CG Johnston, and JA Bumpus (2000) Bioremediation of soil contaminated with explosives at the Naval Weapons Station Yorktown. Soil Sed. Contam. 9:537-548.
Johnston, CG, MA Becerra, RL Lutz, and MA Staton (1997) Fungal remediation of PCP and TNT contaminated soil in the field. In: Papers from the Fourth International In Situ and On Site Bioremediation Symposium (New Orleans, LA). Vol. 2:537-544. Battelle Press, Columbus OH.
Johnston, CG and SD Aust (1994) Detection of Phanerochaete chrysosporium in soil by PCR and restriction enzyme analysis. Appl Environ Microbiol 60:2350-2354.
Johnston, CG and SD Aust (1994) Transcription of ligninase H8 by Phanerochaete chrysosporium under nutrient nitrogen sufficient conditions. Biochem and Biophysical Research Communications.200:108-112.
Recent Published Abstracts
MD Gacura, M. Osama, GP Johnston, RR Riesen, and CG Johnston (In prep.) Effect of organic nitrogen on Polycyclic Aromatic Hydrocarbon (PAH) degradation in contaminated river sediment by Pleurotus ostreatus. ASM Ann Meet, Philadelphia, PA
Z. Kalik, GP Johnston, and CG Johnston (In prep.) Effect of respiratory inhibitors on microbial activity in contaminated river sediment. ASM Ann Meet, Philadelphia, PA
GP Johnston and CG Johnston 2008 Use of Organic Substrates for Remediation of Acid Mine Drainage by Indigenous Sulfate-Reducing Bacteria (SRB): Bench Scale Results. ASM Ann Meet, Boston, MA
GR Bosiljcic, MD Gacura, and CG Johnston 2008 Colonization and Remediation of Polycyclic Aromatic Hydrocarbon (PAH) Contaminated River Sediment using Pleurotus ostreatus. ASM Ann Meet, Boston, MA
GP Basto Salgodo, AMH Ashe, and CG Johnston 2006 Baseline assessment of abandoned mine drainage to evaluate potential remediation utilizing indigenous microbes. OBASM Ann Meet, Canton, OH
GP Basto Salgodo and CG Johnston 2006 "Microbial Particle Factor" as an indicator of fecal pollution in Cattaraugus Creek, NY. ASM Ann Meet, Orlando, FL
JJ Mosher, RH Findlay, and CG Johnston 2004 Physical and chemical factors affecting microbial biomass and activity in contaminated subsurface sediment. Internat Soc Microb Ecol, Cancun, Mexico.